新加坡国立大学黄志伟教授学术报告通知

发布者:材料科学与工程学院、信息科学与纳米技术研究院 发布时间:2019-11-05浏览次数:10

报告题目: Novel coherent Raman scattering microscopy: breaking the diffraction limit for label-free super-resolution biomolecular imaging

报 告 人: 黄志伟 教授

单   位: 新加坡国立大学

时   间:  20191030日下午200

地   点材料学科楼四楼413大会议室

 

报告摘要

Coherent Raman scattering (CRS) (e.g., coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS)) microscopy has emerged as a powerful tool for label-free biomolecular imaging in biological and biomedical systems, but the spatial resolution of CRS microscopy is diffraction limited. In this talk, I will present our very recent work on higher-order coherent anti-Stokes Raman scattering (HO-CARS) microscopy to break the diffraction limit for the first time for label-free super-resolution vibrational imaging. The resolution enhancement of HO-CARS microscopy has been quantitatively analyzed and also demonstrated in biological samples (e.g., Hela cells, buccal cells). HO-CARS technique provides an inherent high resonant to non-resonant (R/NR) background ratio as compared to conventional CARS microscopy. We demonstrate that under tight focusing with high N.A. objective, CARS signal originating from higher-order nonlinear optical processes  dominates over the cascaded lower-order nonlinear optical process , yielding much richer spectroscopic information for tissue and cells characterization. This work illustrates that HO-CARS microscopy can be an appealing tool for label-free super-resolution imaging in biological and biomedical systems with high image contrast.

  

个人简介

Dr Zhiwei Huang is Associate Professor with tenure in Department of Biomedical Engineering (BME), Faculty of Engineering at the National University of Singapore (NUS). He is the Head of Optical Bioimaging Lab at BME/NUS, and a world-renowned expert in biomedical optics and microscopy imaging. His major research areas are in the fields of biomedical optics, microscopy, Raman spectroscopy and imaging, particularly centering on the development of super-resolution microscopy and nonlinear optical microscopy imaging techniques (e.g., coherent Raman scattering microscopy and multiphoton microscopy) and their applications in biomolecular imaging, as well as the development of novel fiber-optic endoscopic imaging and Raman spectroscopy, enabling early diagnosis and detection of epithelial precancer and cancer. He has pioneered in Raman endoscopy and label-free super-resolution bioimaging technologies, and published over 150 peer-reviewed articles in leading journals (e.g., Nature Photonics, Gastroenterology, Nano Letters), and delivered over 100 plenary/keynote/invited talks worldwide. He has filed over 30 US patents with 10 licensed for commercialization. He won many international awards related to novel Raman spectroscopy and imaging techniques. His IMDX technique invented was ranked No.1 among the top 10 medical devices listed in Medica, Germany. He is the node leader in Singapore in Biophotonics4Life (BP4L) Worldwide Consortium, and conference chair for Biomedical Vibrational Spectroscopy Symposium in SPIE-Photonics West-BIOS Conference in San Francisco, USA.